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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence information is just not however out there.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide usually has the epitope recognized from the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best benefits are...
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This guide outlines further more doable optimization of cellular and immunoassay parameters to be certain the absolute best outcomes are...
Antibodies sure to the blocking peptide not bind to your epitope over the focus on protein. By comparing the staining in the blocked antibody compared to the antibody by yourself, you can see which staining is particular.
Goal Information and facts Cyclin E belongs towards the hugely conserved cyclin spouse and children, whose customers are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which lead on the temporal coordination of every mitotic occasion. Cyclin E types a posh with and capabilities being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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